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KMID : 1161420210240070756
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Journal of Medicinal Food
2021 Volume.24 No. 7 p.756 ~ p.761
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Cytotoxicity, Acute, and Subacute Study of Hydroalcoholic Root Extract of Carissa spinarum L. on Wistar Rats
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Dossou-Yovo Komlan M.
Diallo Aboudoulatif
Lawson-Evi Povi
Darre Tchin
Bakoma Batomayena
Eklu-Gadegbeku Kwashie
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Abstract
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Carissa spinarum L. (Apocynaceae) is used traditionally, in Africa, to treat many diseases such as malaria, sickle cell anemia, epilepsy, helminthoses, and sexual weakness. The aim of this study is to investigate the cytotoxicity on Artemia salina, the acute and subacute (28 days) oral toxicity of C. spinarum hydroalcoholic root extract on Wistar rats. The cytotoxicity was performed on A. salina larvae. The acute and subacute toxicity was performed using Organization of Economic Cooperation and Development guideline. Malondiadehyde as lipoperoxidation marker was evaluated and expressed according to tissue proteins. The cytotoxicity has shown that the lethal concentration 50 (LC50) was 0.9?mg/mL. The limit test dose of 5000?mg/kg did not provoke death or toxicity signs. For the subacute toxicity, no signs of toxicity or mortality were observed during the experiment. Results of biochemical and hematological parameters have not shown any treatment-related abnormalities, except a significant decrease of alkaline phosphatase at 1000?mg/kg (P?
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KEYWORD
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acute toxicity, Artemia salina, Carissa spinarum, subacute toxicity, Wistar rats
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